[A morphological study with serial histological slices on the normal and abnormal gubernacula in newborn male mice].
Zhonghua Nan Ke Xue
; 14(3): 206-10, 2008 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-18488330
ABSTRACT
OBJECTIVE:
To explore the feasibility of serial slices microscopic histological investigation for the elaborate evaluation of reproductive system malformations.METHODS:
Newborn male mice prenatally exposed to different doses of subcutaneously given diethylstilbestrol (DES) from gestational day 9 to 17 were treated by fixing parts of the abdomen in situ and setting them to transected serial slices. All the slices were stained, studied under the microscope and serially recorded by software. The gubernaculum was morphologically analyzed and its location and size were measured.RESULTS:
Morphologically, the gubernaculum could be identified clearly, its structure inhomogeneous from proximal to distal and dissymmetric from right to left. The environmental estrogen produced different effects on the morphology of the gubernaculum in different parts and most obviously affected its length.CONCLUSION:
Prenatal exposure to environmental estrogen has evident and general effects on the gubernacular development of newborn male mice. The morphological study with serial histological slices gives a precise and systematic evaluation of genital malformations.
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Base de dados:
MEDLINE
Assunto principal:
Testículo
/
Anormalidades Urogenitais
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China