[The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2].
Bing Du Xue Bao
; 24(2): 83-7, 2008 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-18533338
ABSTRACT
Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systematically study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies(McAbs) which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Anticorpos Anti-Hepatite
/
Vírus da Hepatite E
/
Anticorpos Monoclonais
/
Epitopos
Limite:
Animals
Idioma:
Zh
Revista:
Bing Du Xue Bao
Assunto da revista:
VIROLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China