[Experimental study on thermochemotherapy combined with verapamil for reversing the multidrug resistance responsible to breast cancer cell].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 39(3): 391-3, 2008 May.
Article
em Zh
| MEDLINE
| ID: mdl-18575322
ABSTRACT
OBJECTIVE:
To reverse the multidrug resistance of MCF-7/ADM cell line by thermochemotherapy combined with verapamil (VRP).METHODS:
The drug resistance cells MCF-7/ADM were treated with 3 microg/mL Adriamycin and different concentration VRP (0-5 micromol/L), and then cultured with 37-43 degrees C for 30 minutes. Confocal microscopy was used to demonstrate the fluorescence adriamycin in cells, and the cell apoptosis rate was analyzed with flow cytometry.RESULTS:
With the increases of thermochemical temperature and VRP concentration, the cell adriamycin fluorescence intensity and apoptosis rate of MCF-7/ADM were raised. Thermochemotherapy showed marked synergistic effect with VRP in MCF-7/ADM.CONCLUSION:
It is suggested that thermochemotherapy combined with Verapamil could be used as a way to overcome the multidrug resistance.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Verapamil
/
Doxorrubicina
/
Resistência a Múltiplos Medicamentos
/
Resistencia a Medicamentos Antineoplásicos
/
Temperatura Alta
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China