[Experimental study on thermochemotherapy combined with verapamil for reversing the multidrug resistance responsible to breast cancer cell].

ABSTRACT

OBJECTIVE: To reverse the multidrug resistance of MCF-7/ADM cell line by thermochemotherapy combined with verapamil (VRP). METHODS: The drug resistance cells MCF-7/ADM were treated with 3 microg/mL Adriamycin and different concentration VRP (0-5 micromol/L), and then cultured with 37-43 degrees C for 30 minutes. Confocal microscopy was used to demonstrate the fluorescence adriamycin in cells, and the cell apoptosis rate was analyzed with flow cytometry. RESULTS: With the increases of thermochemical temperature and VRP concentration, the cell adriamycin fluorescence intensity and apoptosis rate of MCF-7/ADM were raised. Thermochemotherapy showed marked synergistic effect with VRP in MCF-7/ADM. CONCLUSION: It is suggested that thermochemotherapy combined with Verapamil could be used as a way to overcome the multidrug resistance.