High confidence determination of specific protein-protein interactions using quantitative mass spectrometry.
Curr Opin Biotechnol
; 19(4): 331-7, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18590817
ABSTRACT
In recent years, interactions between proteins have successfully been determined by mass spectrometry. A limitation of this technology has been the need for extensive purification, which restricts throughput and implies a tradeoff between specificity and the ability to detect weak or transient interactions. Quantitative proteomics sidesteps this problem by directly comparing specific and control pull-downs. Specific interaction partners are revealed by their quantitative ratios rather than by gel-based visualization and can be retrieved from a vast excess of background proteins. This principle is revolutionizing the protein interaction field as demonstrated by recent applications in fields as diverse as tyrosine signaling pathways, cell adhesion, and chromatin biology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
Idioma:
En
Revista:
Curr Opin Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Alemanha