Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig.
J Appl Genet
; 49(3): 263-5, 2008.
Article
em En
| MEDLINE
| ID: mdl-18670063
The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Suínos
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Expressão Gênica
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DNA Topoisomerases Tipo II
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Fator 1 de Elongação de Peptídeos
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Gliceraldeído 3-Fosfato Desidrogenase (NADP/)
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Proteínas de Ligação a DNA
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Hipoxantina Fosforribosiltransferase
Limite:
Animals
Idioma:
En
Revista:
J Appl Genet
Assunto da revista:
GENETICA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
República Tcheca