The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3.
Dev Comp Immunol
; 33(2): 187-97, 2009 Feb.
Article
em En
| MEDLINE
| ID: mdl-18809432
ABSTRACT
L-rhamnose-binding lectins (RBLs) have been isolated from various kinds of fish and invertebrates and interact with various kinds of bacteria, suggesting RBLs are involved in various inflammatory reactions. We investigated the effect of RBLs from chum salmon (Oncorhynchus keta), named CSL1, 2 and 3, on the peritoneal macrophage cell line from rainbow trout (Oncorhynchus mykiss) (RTM5) and an established fibroblastic-like cell line derived from gonadal tissue of rainbow trout (RTG-2). CSLs were bound to the surface of RTM5 and RTG-2 cells and induced proinflammatory cytokines, including IL-1beta1, IL-1beta2, TNF-alpha1, TNF-alpha2 and IL-8 in both cells by recognizing globotriaosylceramide (Gb3). In addition, CSLs had an opsonic effect on RTM5 cells and this effect was significantly inhibited by L-rhamnose, indicating that CSLs enhanced their phagocytosis by binding to Gb3 on cell surfaces. This is the first finding that Gb3 plays a role in innate immunity by cooperating with natural ligands, RBLs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ramnose
/
Antígenos Glicosídicos Associados a Tumores
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Imunidade Inata
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Lectinas
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão