Biocatalytic polyester acrylation--process optimization and enzyme stability.
Biotechnol Bioeng
; 102(3): 693-9, 2009 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-18821636
ABSTRACT
An OH-functional polyester has been acrylated via transesterification of ethyl acrylate, catalyzed by Candida antarctica lipase B (CalB) in two different preparations Novozym 435 and immobilized on Accurel MP1000. The batch process resulted in incomplete acrylation as well as severe degradation of the polyester. A high degree of acrylation was achieved by optimization through the application of low pressure (15 kPa), continuous inflow of ethyl acrylate and continuous distillation to evaporate the by-product, ethanol. The enzyme preparations displayed good stability with half-lives of 180 and 324 h for Novozym 435 and CalB/MP1000, respectively. This translates into product yields of 3600 and 6200 times the weight of the catalyst, indicating that the enzyme will have a marginal impact on the total process cost.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Acrilatos
/
Estabilidade Enzimática
/
Lipase
Idioma:
En
Revista:
Biotechnol Bioeng
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Suécia