A synthetic peptide encompassing the G5 antigenic region of the rabies virus induces high avidity but poorly neutralizing antibody in immunized animals.
Vaccine
; 26(52): 6749-53, 2008 Dec 09.
Article
em En
| MEDLINE
| ID: mdl-18955098
ABSTRACT
The immunization of goats with a synthetic peptide encompassing the G5 antigenic site of the rabies virus surface glycoprotein induces a strong humoral immune response in the absence of a carrier protein. The immunized animals mounted high antibody titers and showed a strong avidity maturation of the B cell immune response to both the G5-peptide and purified surface glycoprotein G. This antibody weakly neutralized rabies virus carrying the G5 epitope but failed to neutralize escape mutants carrying a single point mutation in this epitope. A putative T helper cell epitope, functional in the context of different caprine MHC haplotypes, was identified by structure analysis of the G5-peptide. This striking dichotomy between high titers and antibody of high avidity to the glycoprotein G and poor neutralizing activity strongly suggests that antibody binding assays such as ELISA cannot always reliably predict the neutralizing activity of sera as measured in functional assays.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raiva
/
Vírus da Raiva
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Vacina Antirrábica
/
Imunização
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Anticorpos Antivirais
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Afinidade de Anticorpos
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Antígenos Virais
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Suíça