Influence of phospholipidic microenvironment on the performance of a polypyrrole enzyme electrode.
Talanta
; 41(6): 925-30, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-18966018
ABSTRACT
Enzyme loaded phosphatidylcholine vesicles have been introduced as a new component in the design of sensing layers for the direct coating of an amperometric transducer. For this purpose, choline oxidase was chosen as a model enzyme, and the resulting vesicles were mixed with an amphiphilic pyrrole derivative [12-(pyrrol-1-yl)dodecyltriethylammonium tetrafluoroborate]. A minute amount of this mixture was deposited and dried on a platinum electrode, then electropolymerized. Compared to a similar preparation omitting phospholipid, the sensitivity of the enzyme electrode increased from 0.06 to 0.85 mA .1. mol(-1). The response, obtained in less than 20 sec, was linearly related to the choline concentration within a broader range, extending from 2.5. 10(-7) to 3. 10(-5)M.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Talanta
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França