Peptide antisense nanoparticles.
Proc Natl Acad Sci U S A
; 105(45): 17222-6, 2008 Nov 11.
Article
em En
| MEDLINE
| ID: mdl-19004812
ABSTRACT
We have designed a heterofunctionalized nanoparticle conjugate consisting of a 13-nm gold nanoparticle (Au NP) containing both antisense oligonucleotides and synthetic peptides. The synthesis of this conjugate is accomplished by mixing thiolated oligonucleotides and cysteine-terminated peptides with gold nanoparticles in the presence of salt, which screens interactions between biomolecules, yielding a densely functionalized nanomaterial. By controlling the stoichiometry of the components in solution, we can control the surface loading of each biomolecule. The conjugates are prepared easily and show perinuclear localization and an enhanced gene regulation activity when tested in a cellular model. This heterofunctionalized structure represents a new strategy for preparing nanomaterials with potential therapeutic applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Regulação da Expressão Gênica
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Oligonucleotídeos Antissenso
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Nanopartículas
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Ouro
Limite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos