Early effects of gamma rays and protons on human melanoma cell viability and morphology.
J Microsc
; 232(3): 517-21, 2008 Dec.
Article
em En
| MEDLINE
| ID: mdl-19094032
ABSTRACT
The effects of irradiation with gamma rays and protons on HTB140 human melanoma cell morphology and viability were analyzed. Exponentially growing cells were irradiated close to the Bragg peak maximum of the 62-MeV proton beam, as well as with (60)Co gamma rays, with doses ranging from 8 to 24 Gy. The overall cell morphology was unchanged 6 and 48 h after gamma irradiation, also showing a relatively weak cell-inactivation level. After exposure to proton beam, considerable changes in cell morphology followed by stronger cell inactivation were achieved. Proliferation capacity of irradiated cells significantly decreased in both experimental set-ups. Higher ionization level of protons with respect to gamma rays, representing the main physical difference between these two types of radiation, was also revealed on the cell membrane level through larger pro-apoptotic capacity of protons.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prótons
/
Sobrevivência Celular
/
Linhagem Celular Tumoral
/
Raios gama
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Melanoma
Limite:
Humans
Idioma:
En
Revista:
J Microsc
Ano de publicação:
2008
Tipo de documento:
Article