Determination of main taxoids in Taxus species by microwave-assisted extraction combined with LC-MS/MS analysis.
J Sep Sci
; 32(2): 192-201, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-19107767
ABSTRACT
A method based on microwave-assisted extraction (MAE) has been developed for the determination of paclitaxel and five related taxoids, namely 10-deacetylbaccatin III (10-DAB III), cephalomannine, 10-deacetylpaclitaxel (10-DAT), 7-xyl-10- deacetylpaclitaxel (7-xyl-10-DAT), and 7-epi-10-deacetylpaclitaxel (7-epi-10-DAT) in Taxus species in this study. The influential parameters of the MAE procedure were optimized, and the optimal conditions were as follows extraction solvent 80% ethanol solution, solid/liquid ratio 110 (g/mL), temperature 50 degrees C, and three extraction cycles, each cycle 10 min. The method validation for LC-MS/MS analysis was performed. The LOD and LOQ were 3.16-9.20 and 12.20-30.45 ng/mL, respectively. Repeatability and reproducibility for the six taxiods with RSD ranged from 2.78 to 3.85% and from 5.26 to 6.60%. The recoveries of the method for the six taxoids were 92.6-105.6%. The developed MAE-LC-MS/MS method was also successfully applied to determine the contents of six taxoids in different Taxus species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Taxus
/
Taxoides
/
Espectrometria de Massas em Tandem
/
Micro-Ondas
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2009
Tipo de documento:
Article