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User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.
Schroeder, Hendrik; Adler, Michael; Gerigk, Katrin; Müller-Chorus, Birgit; Götz, Friedrich; Niemeyer, Christof M.
Afiliação
  • Schroeder H; Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.
Anal Chem ; 81(3): 1275-9, 2009 Feb 01.
Article em En | MEDLINE | ID: mdl-19123774
We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Imunoensaio / Técnicas Analíticas Microfluídicas Idioma: En Revista: Anal Chem Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Imunoensaio / Técnicas Analíticas Microfluídicas Idioma: En Revista: Anal Chem Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Alemanha