Protective immunization with homologous and heterologous antigens against Helicobacter suis challenge in a mouse model.
Vaccine
; 27(9): 1416-21, 2009 Feb 25.
Article
em En
| MEDLINE
| ID: mdl-19136039
ABSTRACT
Helicobacter (H.) suis colonizes the stomach of more than 60% of slaughter pigs and is also of zoonotic importance. Recently, this bacterium was isolated in vitro, enabling the use of pure cultures for research purposes. In this study, mice were immunized intranasally or subcutaneously with whole bacterial cell lysate of H. suis or the closely related species H. bizzozeronii and H. cynogastricus, and subsequently challenged with H. suis. Control groups consisted of non-immunized and non-challenged mice (negative control group), as well as of sham-immunized mice that were inoculated with H. suis (positive control group). Urease tests on stomach tissue samples at 7 weeks after challenge infection were negative in all negative control mice, all intranasally immunized mice except one, and in all and 3 out of 5 animals of the H. cynogastricus and H. suis subcutaneously immunized groups, respectively. H. suis DNA was detected by PCR in the stomach of all positive control animals and all subcutaneously immunized/challenged animals. All negative control animals and some intranasally immunized/challenged mice were PCR-negative. In conclusion, immunization using antigens derived from the same or closely related bacterial species suppressed gastric colonization with H. suis, but complete protection was only achieved in a minority of animals following intranasal immunization.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vacinas Bacterianas
/
Infecções por Helicobacter
/
Helicobacter heilmannii
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Bélgica