Structure of immunoglobulin G by scanning tunnelling microscopy.
J Mol Biol
; 221(2): 361-5, 1991 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-1920422
ABSTRACT
Scanning tunnelling microscopy (STM) has been used to examine the shape of individual immunoglobulin G (IgG) molecules deposited onto a graphite surface. IgG was chosen for this study as it has a well-characterized and distinctive three-dimensional structure. The micrographs clearly reveal the IgG molecule as trilobed, corresponding with the known structural organization of IgG. Comparison of these images with the structure of IgG determined by X-ray crystallography shows that the STM images are consistent with the crystal structure. This illustrates that STM is a valuable technique for examining protein structure, allowing rapid determination of the overall molecular shape that is consistent with more established techniques.
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Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido