The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans.
Plant Cell Rep
; 28(5): 769-76, 2009 May.
Article
em En
| MEDLINE
| ID: mdl-19219608
ABSTRACT
We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T(1) soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetolactato Sintase
/
Proteínas de Plantas
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Oryza
/
Glycine max
Idioma:
En
Revista:
Plant Cell Rep
Assunto da revista:
BOTANICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão