Homing of hematopoietic cells to the bone marrow.
J Vis Exp
; (25)2009 Mar 18.
Article
em En
| MEDLINE
| ID: mdl-19295497
ABSTRACT
Homing is the phenomenon whereby transplanted hematopoietic cells are able to travel to and engraft or establish residence in the bone marrow. Various chemomkines and receptors are involved in the homing of hematopoietic stem cells. This paper outlines the classic homing protocol used in hematopoietic stem cell studies. In general this involves isolating the cell population whose homing needs to be investigated, staining this population with a dye of interest and injecting these cells into the blood stream of a recipient animal. The recipient animal is then sacrificed at a pre-determined time after injection and the bone marrow evaluated for the percentage or absolute number of cells which are positive for the dye of interest. In one of the most common experimental schemes, the homing efficiency of hematopoietic cells from two genetically distinct animals (a wild type animal and the corresponding knock-out) is compared. This article describes the hematopoietic cell homing protocol in the framework of such as experiment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
Limite:
Animals
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2009
Tipo de documento:
Article