[Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides].

ABSTRACT

OBJECTIVE: To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique. METHODS: Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis. RESULTS: In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22. CONCLUSION: SELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.