Vaccination strategies to enhance local immunity and protection against Mycobacteriun tuberculosis.
Vaccine
; 27(12): 1816-24, 2009 Mar 13.
Article
em En
| MEDLINE
| ID: mdl-19402204
ABSTRACT
To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1 0401, because CFP10 contains epitopes for DRB1 0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-gamma by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-gamma production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tuberculose Pulmonar
/
Mycobacterium tuberculosis
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos