[Role of focal adhesion kinase in the hypoxia-induced invasion of SMMC-7721 cells].
Zhonghua Gan Zang Bing Za Zhi
; 17(4): 284-7, 2009 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-19403028
ABSTRACT
OBJECTIVE:
To study focal adhesion kinase (FAK) expression in hypoxia-stressed SMMC-7721 cells and the role of FAK expression in the hypoxia-induced invasion of SMMC-7721 cells.METHODS:
SMMC-7721 cells were cultured in 21% O2 or 1% O2. FAK expression was determined by Western blot. The siRNA expression vector pshRNA-FAK targeting to FAK and the control vector pGensil-2 were transfected into SMMC-7721 cells. The hypoxia-induced migration and invasion ability of SMMC-7721 cells transfected with pshRNA-FAK were analyzed. In normoxia, invasion of SMMC-7721 cells transfected with pcDNA3-FAK was analyzed.RESULTS:
The expression of FAK was increased significantly in SMMC-7721 cells 24 h after hypoxia stress (P<0.01). The level of FAK protein was decreased by 74.6%+/-5.1% after the pshRNA-FAK transfection in normoxia and hypoxia. The migration and invasion of SMMC-7721 cells was increased in 1% O2 (P<0.01). However, the migration and invasion of SMMC-7721 cells transfected with pshRNA-FAK was decreased in 1% O2 (P<0.05). Overexpression of FAK significantly stimulated the invasion of SMMC-7721 cells.CONCLUSION:
Up-regulation of FAK may play an important role in the invasion of SMMC-7721 cells induced by hypoxia.
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma Hepatocelular
/
RNA Interferente Pequeno
/
Proteína-Tirosina Quinases de Adesão Focal
/
Neoplasias Hepáticas
/
Hipóxia
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Assunto da revista:
GASTROENTEROLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China