Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis).
Vet Immunol Immunopathol
; 131(3-4): 268-72, 2009 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-19409620
ABSTRACT
The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tartarugas
/
Região de Junção de Imunoglobulinas
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Vet Immunol Immunopathol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China