Moscatilin repressed lipopolysaccharide-induced HIF-1alpha accumulation and NF-kappaB activation in murine RAW264.7 cells.
Shock
; 33(1): 70-5, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-19487987
ABSTRACT
In the present study, we investigated the signaling pathways involved in the inhibition of cyclooxygenase 2 (COX-2) and iNOS by moscatilin under LPS challenge in murine macrophage-derived cell line RAW264.7. The results showed that moscatilin (10-100 microM) had a significant inhibition in a concentration-dependent manner on proinflammatory enzymes (COX-2 and iNOS) expression and macrophage activation under LPS (100 ng/mL) treatment. Hypoxia-inducible factor 1 (HIF-1)alpha was reported to initiate inflammation under cytokine stimulation or hypoxic conditions. In addition, the increase in transcriptional activity and translation process of HIF-1alpha under LPS stimulation resulted in HIF-1alpha accumulation. Moscatilin, a purified compound from Chinese herbs, had a dominant repression on HIF-1alpha expression via down regulating HIF-1alpha mRNA without inhibition of cell viability, translation machinery, or proteasome-mediated degradation of HIF-1alpha. Moreover, the results showed that moscatilin suppressed nuclear translocation of nuclear factor (NF)-kappaB subunits, p65 and p50, and NF-kappaB activity by inhibiting phosphorylation of inhibitor of kappaBalpha. Taken together, we demonstrated that moscatilin inhibited both COX-2 and iNOS expressions after LPS treatment in RAW264.7. Furthermore, the inhibition of moscatilin seemed to be dependent on the repression of HIF-1alpha accumulation and NF-kappaB activation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos de Benzil
/
Lipopolissacarídeos
/
NF-kappa B
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
Limite:
Animals
Idioma:
En
Revista:
Shock
Assunto da revista:
ANGIOLOGIA
/
CARDIOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Taiwan