Endothelial colony-forming cells from patients with chronic myeloproliferative disorders lack the disease-specific molecular clonality marker.
Blood
; 114(14): 3127-30, 2009 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-19628707
ABSTRACT
Two putative types of circulating endothelial progenitor cells have been recently identified in vitro (1) endothelial colony-forming cell (ECFC) and (2) colony-forming unit-endothelial cell (CFU-EC). Only the former is now recognized to belong to endothelial lineage. We have used the ECFC and CFU-EC assays to readdress the issue of the clonal relation between endothelial progenitor cells and hematopoietic stem cells in patients with Philadelphia-positive and Philadelphia-negative chronic myeloproliferative disorders. Both ECFCs and CFU-ECs were cultured from peripheral blood mononuclear cells, and either BCR-ABL rearrangement or JAK2-V617F mutation were assessed in both types of endothelial colonies. We found that ECFCs lack the disease-specific markers, which are otherwise present in CFU-ECs, thus reinforcing the concept that the latter belongs to the hematopoietic lineage, and showing that in chronic myeloproliferative disorders the cell that gives rise to circulating ECFC has a distinct origin from the cell of the hematopoietic malignant clone.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
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Células-Tronco Hematopoéticas
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Biomarcadores Tumorais
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Proteínas de Fusão bcr-abl
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Células Endoteliais
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Janus Quinase 2
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Transtornos Mieloproliferativos
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Blood
Ano de publicação:
2009
Tipo de documento:
Article