Tissue-spanning redox gradient-dependent assembly of native human papillomavirus type 16 virions.
J Virol
; 83(20): 10515-26, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19656879
ABSTRACT
Papillomavirus capsids are composed of 72 pentamers reinforced through inter- and intrapentameric disulfide bonds. Recent research suggests that virus-like particles and pseudovirions (PsV) can undergo a redox-dependent conformational change involving disulfide interactions. We present here evidence that native virions exploit a tissue-spanning redox gradient that facilitates assembly events in the context of the complete papillomavirus life cycle. DNA encapsidation and infectivity titers are redox dependent in that they can be temporally modulated via treatment of organotypic cultures with oxidized glutathione. These data provide evidence that papillomavirus assembly and maturation is redox-dependent, utilizing multiple steps within both suprabasal and cornified layers.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírion
/
Queratinócitos
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Capsídeo
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Montagem de Vírus
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Papillomavirus Humano 16
Limite:
Humans
Idioma:
En
Revista:
J Virol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos