Biological and molecular characterization of a begomovirus associated with yellow mosaic vein mosaic disease of pumpkin from Northern India.
Virus Genes
; 39(3): 359-70, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19705268
ABSTRACT
The biological and molecular properties of Squash leaf curl China virus from Varanasi, India (SLCCNV-IN[INVarPum]) were characterized. SLCCNV-IN[INVarPum] could be transmitted by grafting and through whitefly transmission. The complete DNA-A and DNA-B components were amplified through PCR using specific DNA-A and DNA-B primers. The DNA-A of the isolate was comprised of 2,738 nucleotides, encoding typical six open reading frames, and DNA-B of 2,704 nucleotides, encoding two ORFs. Genome organization of the isolate was typical of an old world bipartite begomovirus. Comparisons showed that DNA-A and its intergenic region have the highest sequence identity (97.6 and 97.4%, respectively) with the SLCCNV-IN[INLucPum]; (DQ026296). This data suggested that the isolate is a same begomovirus species for which the name Squash leaf curl China virus-India[IndiaVaranasiPumpkin] is proposed. DNA-B showed maximum sequence identity (89.2%) with SLCCNV-IN[INCoiPum] (AY184488). Phylogenetic analysis of the present isolate showed close relationship to other cucurbit-infecting geminiviruses. This is the first evidence of occurrence of the bipartite Squash leaf curl China virus associated with severe yellow mosaic disease of pumpkin in northern India.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Cucurbita
/
Begomovirus
Tipo de estudo:
Risk_factors_studies
País/Região como assunto:
Asia
Idioma:
En
Revista:
Virus Genes
Assunto da revista:
BIOLOGIA MOLECULAR
/
VIROLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Índia