Tales of poly(A): a review.
Gene
; 91(2): 151-8, 1990 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-1976572
Until recently, evidence to support a translational role for the 3'-poly(A) tract of eukaryotic mRNAs has been mostly indirect, including: a correlation between the adenylation status of individual mRNAs and their translatability in vivo or in vitro, the demonstration that exogenously added poly(A) is a potent competitive inhibitor of the translation of poly(A)+mRNA, but not poly(A)-mRNAs in vitro, and a correlation between the abundance and stability of poly(A)-binding proteins (PABPs) and the rate of translational initiation in vivo. However, more recent studies demonstrate directly that poly(A)+mRNAs can initiate translation more efficiently than poly(A)-mRNAs, and indicate that this effect is: (i) targeted to the formation of 80S initiation complexes, and (ii) likely to be mediated by the cytoplasmic PABP. We suggest that the 3'-poly(A) tail should be considered a translational enhancer which may stimulate translational initiation in much the same way that transcriptional enhancers are thought to stimulate transcriptional initiation.
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Base de dados:
MEDLINE
Assunto principal:
Poli A
/
Biossíntese de Proteínas
/
RNA Mensageiro
Limite:
Animals
Idioma:
En
Revista:
Gene
Ano de publicação:
1990
Tipo de documento:
Article