Enrichment of glycopeptides for glycan structure and attachment site identification.
Nat Methods
; 6(11): 809-11, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19838169
ABSTRACT
We present a method to enrich for glycoproteins from proteomic samples. Sialylated glycoproteins were selectively periodate-oxidized, captured on hydrazide beads, trypsinized and released by acid hydrolysis of sialic acid glycosidic bonds. Mass spectrometric fragment analysis allowed identification of glycan structures, and additional fragmentation of deglycosylated ions yielded peptide sequence information, which allowed glycan attachment site and protein identification. We identified 36 N-linked and 44 O-linked glycosylation sites on glycoproteins from human cerebrospinal fluid.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas
/
Proteínas do Líquido Cefalorraquidiano
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Suécia