Presence and expression of the mating type locus in Paracoccidioides brasiliensis isolates.
Fungal Genet Biol
; 47(4): 373-80, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-19932183
ABSTRACT
Paracoccidioides brasiliensis has been classified in the phylum Ascomycota, order Onygenales, family Ajellomycetaceae, even in the absence of a known sexual cycle or mating system. The objective of this work was to determine the presence of the mating type locus in 71 P. brasiliensis isolates from various sources. A PCR assay using specific primers for the MAT 1 gene was developed and applied for the detection of such genes. Two heterothallic groups (MAT1-1 or MAT1-2) were recognized and, in some isolates, gene expression was confirmed indicating the existence of a basal gene expression. The distribution of two mating type loci in the studied population suggested that sexual reproduction might occur in P. brasiliensis. This finding points towards the possibility of applying a more precise definition of the concept of biological species to P. brasiliensis. Further studies should be conducted to confirm the sexual capacity of this fungus and its implications among phylogenetic species and geographical distribution.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Paracoccidioides
/
Expressão Gênica
/
Genes Fúngicos Tipo Acasalamento
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Fungal Genet Biol
Assunto da revista:
GENETICA
/
MICROBIOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Colômbia