GyrA interacts with MarR to reduce repression of the marRAB operon in Escherichia coli.
J Bacteriol
; 192(4): 942-8, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-19933356
ABSTRACT
Bacterial two-hybrid studies of randomly cloned Escherichia coli DNA identified a physical interaction between GyrA, subunit A of gyrase, and MarR, a repressor of the marRAB operon. GyrA-His immobilized on Ni-nitrilotriacetic acid (NiNTA) resin bound MarR, while MarR alone did not bind. GyrA interfered with MarR binding to marO, as detected by electrophoretic mobility assays. In a strain bearing the marRAB operon and a marO-lacZ reporter, overexpression of GyrA increased LacZ activity, indicating decreased repression of marO-lacZ by MarR. These results were confirmed by an increased survival of cells treated with quinolones and other antibiotics when GyrA was overexpressed. This work, like a previous study examining TktA (12), shows that unrelated proteins can regulate MarR activity. The findings reveal an unexpected regulatory function of GyrA in antibiotic resistance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Regulação Bacteriana da Expressão Gênica
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DNA Girase
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Proteínas de Escherichia coli
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Mapeamento de Interação de Proteínas
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Escherichia coli
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos