Development of TaqMan probe-based real-time PCR method for erm(A),erm(B), and erm(C), rapid detection of macrolide-lincosamide-streptogramin B resistance genes, from clinical isolates.
J Microbiol Biotechnol
; 19(11): 1464-9, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19996702
ABSTRACT
To achieve more accurate and rapid detection of macrolidelincosamide- streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Sondas de DNA
/
Reação em Cadeia da Polimerase
/
Infecção Hospitalar
/
Infecções por Bactérias Gram-Positivas
/
Bactérias Gram-Positivas
/
Metiltransferases
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Microbiol Biotechnol
Ano de publicação:
2009
Tipo de documento:
Article