Photosynthetic performance and fertility are repressed in GmAOX2b antisense soybean.
Plant Physiol
; 152(3): 1638-49, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-20097793
ABSTRACT
The alternative oxidase (AOX) is a cyanide-resistant oxidase that provides an alternative outlet for electrons from the respiratory electron transport chain embedded in the inner membrane of plant mitochondria. Examination of soybean (Glycine max) plants carrying a GmAOX2b antisense gene showed AOX to have a central role in reproductive development and fecundity. In three independently transformed antisense lines, seed set was reduced by 16% to 43%, whereas ovule abortion increased by 1.2- to 1.7-fold when compared with nontransgenic transformation control plants. Reduced fecundity was associated with reductions in whole leaf cyanide-resistant, salicylhydroxamic acid-sensitive respiration and net photosynthesis, but there was no change in total respiration in the dark. The frequency of potential fertilization events was reduced by at least one-third in the antisense plants as a likely consequence of prefertilization defects. Pistils of the antisense plants contained a higher proportion of immature-sized, nonfertile embryo sacs compared with nontransgenic control plants. Increased rates of pollen abortion in vivo and reduced rates of pollen germination in vitro suggested that the antisense gene compromised pollen development and function. Reciprocal crosses between antisense and nontransgenic plants revealed that pollen produced by antisense plants was less active in fertilization. Taken together, the results presented here indicate that AOX expression has an important role in determining normal gametophyte development and function.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
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Fotossíntese
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Proteínas de Plantas
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Glycine max
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Fertilidade
Idioma:
En
Revista:
Plant Physiol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Austrália