(-)-Hinokinin-loaded poly(D,-lactide-co-glycolide) microparticles for Chagas disease.
Parasitol Res
; 106(3): 703-8, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-20107838
ABSTRACT
The (-)-hinokinin display high activity against Trypanosoma cruzi in vitro and in vivo. (-)-Hinokinin-loaded poly(D,L-lactide-co-glycolide) microparticles were prepared and characterized in order to protect (-)-hinokinin of biological interactions and promote its sustained release for treatment of Chagas disease. The microparticles contain (-)-hinokinin were prepared by the classical method of the emulsion/solvent evaporation. The scanning electron microscopy, light-scattering analyzer were used to study the morphology and particle size, respectively. The encapsulation efficiency was determined, drug release studies were kinetically evaluated, and the trypanocidal effect was evaluated in vivo. (-)-Hinokinin-loaded microparticles obtained showed a mean diameter of 0.862 microm with smooth surface and spherical shape. The encapsulation efficiency was 72.46 +/- 2.92% and developed system maintained drug release with Higuchi kinetics. The preparation method showed to be suitable, since the morphological characteristics, encapsulation efficiency, and in vitro release profile were satisfactory. In vivo assays showed significant reduction of mice parasitaemia after administration of (-)-hinokinin-loaded microparticles. Thus, the developed microparticles seem to be a promising system for sustained release of (-)-hinokinin for treatment of Chagas disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Poliglicólico
/
4-Butirolactona
/
Doença de Chagas
/
Lignanas
/
Ácido Láctico
/
Preparações de Ação Retardada
/
Dioxóis
/
Microesferas
/
Antiprotozoários
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Parasitol Res
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Brasil