Application of gel permeation HPLC for lipoprotein profiling in dogs.
J Vet Med Sci
; 72(6): 813-7, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-20145378
ABSTRACT
A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28-29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.
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Base de dados:
MEDLINE
Assunto principal:
Cães
/
Lipoproteínas
Limite:
Animals
Idioma:
En
Revista:
J Vet Med Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão