A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA.
Bioorg Med Chem Lett
; 20(7): 2350-3, 2010 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-20206514
ABSTRACT
Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
DNA Polimerase Dirigida por RNA
/
Regiões não Traduzidas
/
Quadruplex G
Limite:
Humans
Idioma:
En
Revista:
Bioorg Med Chem Lett
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão