RNAi knockdown of a salivary transcript leading to lethality in the pea aphid, Acyrthosiphon pisum.
J Insect Sci
; 6: 1-7, 2006.
Article
em En
| MEDLINE
| ID: mdl-20233093
ABSTRACT
Abstract Injection of siRNA (small interfering RNA) into parthenogenetic adult pea aphids (Acyrthosiphon pisum) is shown here to lead to depletion of a target salivary gland transcript. The siRNA was generated from double stranded RNA that covered most of the open reading frame of the transcript, which we have called Coo2. The Coo2 transcript level decreases dramatically over a 3-day period after injection of siRNA. With a lag of 1 to 2 days, the siCoo2-RNA injected insects died, on average 8 days before the death of control insects injected with siRNA for green fluorescent protein. It appears, therefore, that siRNA injections into adults will be a useful tool in studying the roles of individual transcripts in aphid salivary glands and suggests that siCoo2-RNA injections can be a useful positive control in such studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Afídeos
/
Interferência de RNA
/
Técnicas de Silenciamento de Genes
Limite:
Animals
Idioma:
En
Revista:
J Insect Sci
Assunto da revista:
BIOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos