Your browser doesn't support javascript.
loading
HTS-driven discovery of new chemotypes with West Nile Virus inhibitory activity.
Chung, Dong Hoon; Jonsson, Colleen B; Maddox, Clinton; McKellip, Sara N; Moore, Blake P; Heil, Marintha; White, E Lucile; Ananthan, Subramaniam; Li, Qianjun; Feng, Shuang; Rasmussen, Lynn.
Afiliação
  • Chung DH; Department of Biochemistry and Molecular Biology, Southern Research Institute, 2000 9th Ave S. Birmingham, AL 35205, USA. dhchun01@louisville.edu
Molecules ; 15(3): 1690-704, 2010 Mar 12.
Article em En | MEDLINE | ID: mdl-20336008
West Nile virus (WNV) is a positive sense, single-stranded RNA virus that can cause illness in humans when transmitted via mosquito vectors. Unfortunately, no antivirals or vaccines are currently available, and therefore efficient and safe antivirals are urgently needed. We developed a high throughput screen to discover small molecule probes that inhibit virus infection of Vero E6 cells. A primary screen of a 13,001 compound library at a 10 microM final concentration was conducted using the 384-well format. Z' values ranged from 0.54-0.83 with a median of 0.74. Average S/B was 17 and S/N for each plate ranged from 10.8 to 23.9. Twenty-six compounds showed a dose response in the HT screen and were further evaluated in a time of addition assay and in a titer reduction assay. Seven compounds showed potential as small molecule probes directed at WNV. The hit rate from the primary screen was 0.185% (24 compounds out of 13,001 compounds) and from the secondary screens was 0.053% (7 out of 13,001 compounds) respectively.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Antivirais / Vírus do Nilo Ocidental Limite: Animals Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Antivirais / Vírus do Nilo Ocidental Limite: Animals Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Estados Unidos