Bacteriophage lambda: a paradigm revisited.
J Virol
; 84(13): 6876-9, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20375161
Bacteriophage lambda has an archetypal immunity system, which prevents the superinfection of its Escherichia coli lysogens. It is now known that superinfection can occur with toxigenic lambda-like phages at a high frequency, and here we demonstrate that the superinfection of a lambda lysogen can lead to the acquisition of additional lambda genomes, which was confirmed by Southern hybridization and quantitative PCR. As many as eight integration events were observed but at a very low frequency (6.4 x 10(-4)) and always as multiple insertions at the established primary integration site in E. coli. Sequence analysis of the complete immunity region demonstrated that these multiply infected lysogens were not immunity mutants. In conclusion, although lambda superinfection immunity can be confounded, it is a rare event.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Superinfecção
/
Integração Viral
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Bacteriófago lambda
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Prófagos
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Escherichia coli
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Lisogenia
Idioma:
En
Revista:
J Virol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido