7-ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages.
Acta Med Okayama
; 64(2): 85-93, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-20424663
ABSTRACT
We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.
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Base de dados:
MEDLINE
Assunto principal:
NF-kappa B
/
Apoptose
/
Cetocolesteróis
/
Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Acta Med Okayama
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China