Production of recombinant miraculin using transgenic tomatoes in a closed cultivation system.
J Agric Food Chem
; 58(10): 6096-101, 2010 May 26.
Article
em En
| MEDLINE
| ID: mdl-20426470
ABSTRACT
We constructed a cultivation system with a controlled light period, light intensity, temperature, and CO(2) concentration for mass production of the taste-modifying protein miraculin from transgenic tomatoes. The tomato plants exhibited normal growth and produced over 270 g of fresh weight (FW) fruit per plant, with the recombinant miraculin concentration reaching up to 90 microg per g FW of tomatoes. The recombinant miraculin content of transgenic tomatoes was compared to that of plants grown in a netted greenhouse. The recombinant miraculin content of transgenic tomatoes grown in a closed cultivation system was more stable than that of tomatoes grown in a netted greenhouse, suggesting that the closed cultivation system is suitable for the production of recombinant miraculin. We estimate that 45 tFW of tomatoes and 4 kg of recombinant miraculin per 1,000 m(2) of cultivation area can be harvested per year.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Glicoproteínas
/
Plantas Geneticamente Modificadas
/
Solanum lycopersicum
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão