[Development of the real-time reverse transcription-polymerase chain reaction assay for the rapid detection of rubella virus].
Zhongguo Yi Miao He Mian Yi
; 16(1): 25-9, 2010 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-20450068
ABSTRACT
OBJECTIVE:
A new TaqMan probe based real-time assay was developed to rapid detection of rubella virus.METHODS:
The specific primer pair and probe were designed within the conserved P150 gene of rubella virus and the PCR reactive condition was optimized to improve the sensitivity and specificity of the assay. Clinical specimens collected from two outbreaks were detected by the developed assay.RESULTS:
The assay is specific to detect rubella virus. There is no cross-reactions to measles, mumps, influenza and other respiratory viruses. It could detect 0.01 TCID50/tube of rubella RNA and took only three hours to finish a detection. In addition, the assay is simple, accurate and repeatable.CONCLUSION:
The TaqMan based real-time PCR developed in this study provides a fast, sensitive and specific tool for molecular diagnosis on rubella virus.
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Base de dados:
MEDLINE
Assunto principal:
Vírus da Rubéola
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Yi Miao He Mian Yi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China