Genetic diversity of Greek Aegilops species using different types of nuclear genome markers.
Mol Phylogenet Evol
; 56(3): 951-61, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20450978
ABSTRACT
Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) analyses were used to evaluate genetic variability and relationships of Greek Aegilops species. Thirty-eight accessions of seven Greek Aegilops species [Ae. triuncialis (genome UC), Ae. neglecta (UM), Ae. biuncialis (UM), Ae. caudata (C), Ae. comosa (M), Ae. geniculata (MU) and Ae. umbellulata (U)] as well as Triticum accessions were studied. Nineteen RAPD and ten ISSR primers yielded 344 and 170 polymorphic bands, respectively, that were used for the construction of dendrograms. Regardless of the similarity coefficient and marker type used, UPGMA placed 38 Aegilops accessions into one branch while the other branch consisted of wheat species. Within the Aegilops cluster, subgroups were identified that included species that shared the same genome or belonged to the same botanical section. Within the Triticum cluster, two robust subgroups were formed, one including diploid wheat and another including polyploid wheat. In conclusion, results showed that there is genetic diversity in the Greek Aegilops species studied, and clustering based on genetic similarities was in agreement with botanical classifications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Variação Genética
/
Genoma de Planta
/
Poaceae
Tipo de estudo:
Prognostic_studies
País/Região como assunto:
Europa
Idioma:
En
Revista:
Mol Phylogenet Evol
Assunto da revista:
BIOLOGIA
/
BIOLOGIA MOLECULAR
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Grécia