Myosin II contributes to cell-scale actin network treadmilling through network disassembly.
Nature
; 465(7296): 373-7, 2010 May 20.
Article
em En
| MEDLINE
| ID: mdl-20485438
ABSTRACT
Crawling locomotion of eukaryotic cells is achieved by a process dependent on the actin cytoskeleton protrusion of the leading edge requires assembly of a network of actin filaments, which must be disassembled at the cell rear for sustained motility. Although ADF/cofilin proteins have been shown to contribute to actin disassembly, it is not clear how activity of these locally acting proteins could be coordinated over the distance scale of the whole cell. Here we show that non-muscle myosin II has a direct role in actin network disassembly in crawling cells. In fish keratocytes undergoing motility, myosin II is concentrated in regions at the rear with high rates of network disassembly. Activation of myosin II by ATP in detergent-extracted cytoskeletons results in rear-localized disassembly of the actin network. Inhibition of myosin II activity and stabilization of actin filaments synergistically impede cell motility, suggesting the existence of two disassembly pathways, one of which requires myosin II activity. Our results establish the importance of myosin II as an enzyme for actin network disassembly; we propose that gradual formation and reorganization of an actomyosin network provides an intrinsic destruction timer, enabling long-range coordination of actin network treadmilling in motile cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Actinas
/
Miosina Tipo II
/
Células Epiteliais
Limite:
Animals
Idioma:
En
Revista:
Nature
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos