Detection of meticillin-resistant staphylococcus aureus (MRSA) colonization in newborn infants using real-time polymerase chain reaction (PCR).
Acta Paediatr
; 99(11): 1691-4, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-20528798
ABSTRACT
OBJECTIVE:
Meticillin-resistant staphylococcus aureus (MRSA) colonization on neonatal units is a common and important clinical problem. Effectiveness of polymerase chain reaction (PCR) for detecting MRSA nasal colonization of infants was evaluated and compared to culture-based methods. The effect of skin decolonization in affected infants was studied.METHODS:
Paired nasal swabs were collected from infants in our neonatal unit over a 12-month period (September 2007-2008). Colonization with MRSA was determined with a commercially available PCR method and compared to culture.RESULTS:
A total of 696 paired nasal swabs were taken. Three infants were colonized at the beginning and were included. There were positive PCRs in 12 infants. Five infants cultured MRSA from a nasal swab at the same time. No infants were culture-positive when PCR was negative (sensitivity 100%, specificity 99% compared to culture). PCR results were available within 24 h. Five infants were PCR+ and isolated meticillin-sensitive Staphylococcus aureus. This organism gave a false-positive PCR result. Two infants transferred in on broad-spectrum antibiotics were PCR+ and negative by culture. Decolonization led to negative nasal PCR and culture in 4/5 infants to discharge.CONCLUSIONS:
PCR methods are sensitive and specific for detection of MRSA colonization in newborn infants of all gestations with results 1-2 days before culture.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções Estafilocócicas
/
Reação em Cadeia da Polimerase
/
Staphylococcus aureus Resistente à Meticilina
/
Mucosa Nasal
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Female
/
Humans
/
Male
/
Newborn
Idioma:
En
Revista:
Acta Paediatr
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido