Inhibition of p38 MAPK-dependent bronchial contraction after ozone by corticosteroids.
Eur Respir J
; 37(4): 933-42, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-20693246
ABSTRACT
We determined the role of p38 mitogen-activated protein kinase (MAPK) in the increased airway smooth muscle (ASM) contractile responses following ozone and modulation by corticosteroids. Mice were exposed to air or ozone (3 ppm for 3 h) and isometric contractile responses of bronchial rings to acetylcholine (ACh) were measured using a myograph in the presence of p38 MAPK inhibitor, SB239063 (10â»6 M) or dexamethasone (10â»6 M). Because MAPK phosphatase (MKP)-1 is a negative regulator of p38 MAPK, we also studied these effects in MKP-1(-/-) mice. Bronchial rings from ozone-exposed wild-type and MKP-1(-/-) mice showed increased contractile responses, with a leftward shift of the dose-response curve in MKP-1(-/-) mice. SB239063 inhibited bronchial contraction equally in air- and ozone-exposed C57/BL6 and MKP-1(-/-) mice. Dexamethasone inhibited ACh-induced bronchial contraction in both air- and ozone-exposed C57/BL6 mice, but not in air- or ozone-exposed MKP-1(-/-) mice. ACh-stimulated p38 MAPK and heat shock protein (HSP)27 phosphorylation, as measured by Western blotting, and this effect was suppressed by SB239063 in C57/BL6 and MKP-1(-/-) mice, but not by dexamethasone in either air- or ozone-exposed MKP-1(-/-) mice. p38 MAPK plays a role in maximal ACh-induced isometric contractile responses and increased contractility induced by ozone. Dexamethasone inhibits ACh-induced ASM contraction through phosphorylation of p38 MAPK and HSP27.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ozônio
/
Brônquios
/
Corticosteroides
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Proteínas Quinases p38 Ativadas por Mitógeno
Limite:
Animals
/
Humans
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Male
Idioma:
En
Revista:
Eur Respir J
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China