The expression patterns of a eukaryotic initiation factor 3 subunit H in the silk glands in Bombyx mori.
Arch Insect Biochem Physiol
; 75(1): 1-12, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20734419
ABSTRACT
Eukaryotic initiation factor 3 subunit H has been characterized in many organisms, and it has been found to play many roles including help regulate translation initiation. In this work, we studied the tissue distribution and expression profiles of Bombyx mori (B. mori) eIF3 subunit H (BmeIF3h). BmeIF3h was prominently expressed in silk glands, with anterior silk glands (ASGs), middle silk glands (MSGs), and posterior silk glands (PSGs) all expressing BmeIF3h. The expression levels of BmeIF3h in MSGs and PSGs were higher than that in ASGs during 0 d and 2 d of the 5th instar larvae. The expression levels of BmeIF3h in MSGs and PSGs were up-regulated once the silk glands began to synthesize silk protein during the feeding stage of the 4th instar larvae. Immunohistochemistry showed that BmeIF3h was distributed in the cytoplasm of MSGs cells and in both the nucleus and the cytoplasm of PSGs cells. These data suggest that BmeIF3h had different action behaviors in the MSGs and PSGs related to the production of the silk glue proteins and silk fibre proteins, respectively.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bombyx
/
Fator de Iniciação 3 em Eucariotos
Limite:
Animals
Idioma:
En
Revista:
Arch Insect Biochem Physiol
Assunto da revista:
BIOLOGIA
/
BIOQUIMICA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China