One-step multiplex reverse-transcriptase PCR for detecting pandemic (H1N1) 2009 influenza virus.
J Vet Med Sci
; 73(1): 55-63, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-20798477
ABSTRACT
Pandemic (H1N1) 2009 influenza has spread throughout the world since April 2009 and has caused many human deaths since its first report in humans. Pandemic (H1N1) 2009 influenza virus was first identified in a Canadian pig herd in April 2009 and has been reported in more than ten countries, including Korea. We developed a one-step multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay based on the matrix gene that discriminates pandemic (H1N1) 2009 influenza virus from endemic swine influenza viruses. The sensitivity of this assay was 100 copies of in vitro-transcribed target RNA and 0.01 tissue culture infective dose (TCID(50)/ml) of virus and was as high as those of conventional influenza A virus common matrix reverse transcriptase PCR assays and real-time reverse transcriptase PCR assays (1 to 200 copies) developed for detecting pandemic (H1N1) 2009 influenza viruses from human and pig samples. This one-step multiplex RT-PCR assay would be a good tool in monitoring pandemic (H1N1) 2009 influenza virus among pig herds on a regular basis.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Orthomyxoviridae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Influenza A Subtipo H1N1
/
Pandemias
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Vet Med Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2011
Tipo de documento:
Article