Expression, functional, and structural analysis of proteins critical for otoconia development.
Dev Dyn
; 239(10): 2659-73, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-20803598
ABSTRACT
Otoconia, developed during late gestation and perinatal stages, couple mechanic force to the sensory hair cells in the vestibule for motion detection and bodily balance. In the present work, we have investigated whether compensatory deposition of another protein(s) may have taken place to partially alleviate the detrimental effects of Oc90 deletion by analyzing a comprehensive list of plausible candidates, and have found a drastic increase in the deposition of Sparc-like 1 (aka Sc1 or hevin) in Oc90 null versus wt otoconia. We show that such up-regulation is specific to Sc1, and that stable transfection of Oc90 and Sc1 full-length expression constructs in NIH/3T3 cells indeed promotes matrix calcification. Analysis and modeling of Oc90 and Sc1 protein structures show common features that may be critical requirements for the otoconial matrix backbone protein. Such information will serve as the foundation for future regenerative purposes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Membrana dos Otólitos
Limite:
Animals
Idioma:
En
Revista:
Dev Dyn
Assunto da revista:
ANATOMIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos