Morphological score assignment guidelines for the dechorionated zebrafish teratogenicity assay.
Birth Defects Res B Dev Reprod Toxicol
; 89(5): 382-95, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-20836125
ABSTRACT
BACKGROUND:
Recently we reported the development and optimization of a zebrafish teratogenicity assay using dechorionated AB strain embryos, a promising assay that was 87% concordant in correctly identifying in vivo teratogens and non-teratogens from a set of 31 compounds (Brannen et al., 2010 Birth Defects Res 8966-77).METHODS:
This assay utilizes a zebrafish morphological score system to characterize adverse effects and identify the no-observed-adverse-effect level (NOAEL).RESULTS:
This report describes in detail the morphological score system used in the dechorionated zebrafish embryo culture teratogenicity assay. The morphological assessment includes evaluation of most structures and organ systems and grades relative severity of abnormalities.CONCLUSIONS:
To this end, the morphological score system provides information of tissue-specific teratogenicity that has been found to have good concordance with structures found affected in vivo and can also be used to rank compounds based on the severity of malformations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Teratogênicos
/
Peixe-Zebra
/
Técnicas de Cultura Embrionária
/
Embrião não Mamífero
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Birth Defects Res B Dev Reprod Toxicol
Assunto da revista:
TERATOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos