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Simultaneous determination of m-nisoldipine and its three metabolites in rat plasma by liquid chromatography-mass spectrometry.
Kong, Dezhi; Li, Sanni; Zhang, Xiaowei; Gu, Jianmin; Liu, Man; Meng, Yan; Fu, Yan; La, Xiaojin; Xue, Gangqiang; Zhang, Lantong; Wang, Qiao.
Afiliação
  • Kong D; Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China.
J Chromatogr B Analyt Technol Biomed Life Sci ; 878(29): 2989-96, 2010 Nov 01.
Article em En | MEDLINE | ID: mdl-20870469
A simple, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of m-nisoldipine and its three metabolites in rat plasma has been developed using nitrendipine as an internal standard (IS). Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse phase C(18) column and analyzed by MS in the multiple reaction monitoring (MRM) mode. To avoid contamination by residual sample in the injection syringe, a special injection protocol was developed. We found that m-nisoldipine, metabolite M1 and IS could be ionized under positive or negative electrospray ionization conditions, whereas metabolite M and M2 could only be ionized in the positive mode. The mass spectrometry fragmentation pathways for these analytes are analyzed and discussed herein. The total analysis time required less than 5 min per sample. We employed this method successfully to study the metabolism of m-nisoldipine when it was orally administered to rats at a dose of 9 mg/kg. Three metabolites of m-nisoldipine and an unknown compound of molecular weight 386 were found for the first time in rat plasma. The concentration of the potentially active metabolite was approximately equal to its parent compound concentration.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nisoldipino / Cromatografia Líquida / Espectrometria de Massas em Tandem Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nisoldipino / Cromatografia Líquida / Espectrometria de Massas em Tandem Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China