Heat shock causes the collapse of the intermediate filament cytoskeleton in Drosophila embryos.
Dev Genet
; 11(4): 270-9, 1990.
Article
em En
| MEDLINE
| ID: mdl-2090374
ABSTRACT
Heat shock has a dramatic effect on the organization of the cytoplasm, causing the intermediate filament cytoskeleton to aggregate at the nucleus. This has previously been shown in cultured Drosophila and mammalian cells. In this paper we analyze the heat lability of the intermediate filament cytoskeleton in early Drosophila embryos by indirect immunofluorescence. At all stages of embryogenesis tested, the intermediate filament cytoskeleton, which is maternally provided, is severely disturbed by 30 min heat shock at 37 degrees C. After the nuclei have migrated to the subcortical cytoplasm, it collapses around them. Nuclei in all heat-shocked embryos are considerably enlarged and become displaced. Embryos before cellular blastoderm stage, in which heat shock protein synthesis is not inducible, are irreversibly arrested in development by heat shock. Embryos at or after cellular blastoderm, which do synthesize heat shock proteins in response to stress, are also immediately arrested in development but continue development when returned to 25 degrees C. We discuss the possibility that cytoplasmic events such as the intermediate filament cytoskeleton rearrangement may be involved in heat shock-mediated phenocopy induction.
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Base de dados:
MEDLINE
Assunto principal:
Filamentos Intermediários
/
Drosophila
/
Proteínas de Filamentos Intermediários
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Dev Genet
Ano de publicação:
1990
Tipo de documento:
Article